Morphology and genetic analysis of <i>Coolia malayensis</i> (Dinophyceae) from the North Viet Nam

Phan Tan Luom; Huynh Thi Ngoc Duyen; Tran Thi Minh Hue; Pham Xuan Ky; Nguyen Xuan Vy; Dao Viet Ha

doi:10.15625/2525-2518/20664

Author affiliations

Authors

Phan Tan Luom Institute of Oceanography, Vietnam Academy of Science and Technology, 01 Cau Da Street, Nha Trang Ward, Khanh Hoa, Viet Nam
Huynh Thi Ngoc Duyen Institute of Oceanography, Vietnam Academy of Science and Technology, 01 Cau Da Street, Nha Trang Ward, Khanh Hoa, Viet Nam
Tran Thi Minh Hue Institute of Oceanography, Vietnam Academy of Science and Technology, 01 Cau Da Street, Nha Trang Ward, Khanh Hoa, Viet Nam
Pham Xuan Ky Institute of Oceanography, Vietnam Academy of Science and Technology, 01 Cau Da Street, Nha Trang Ward, Khanh Hoa, Viet Nam
Nguyen Xuan Vy Institute of Oceanography, Vietnam Academy of Science and Technology, 01 Cau Da Street, Nha Trang Ward, Khanh Hoa, Viet Nam
Dao Viet Ha Institute of Oceanography, Vietnam Academy of Science and Technology, 01 Cau Da Street, Nha Trang Ward, Khanh Hoa, Viet Nam https://orcid.org/0000-0002-4996-501X

DOI:

https://doi.org/10.15625/2525-2518/20664

Keywords:

Coolia malayensis, Gulf of Tonkin, LSU, morphology, Viet Nam

Abstract

In this study, two strains of Coolia malayensis isolated from macroalgae Padina spp. and Sargassum spp. collected in the Gulf of Tonkin, Viet Nam were identified and described using morphological characteristics and phylogenetic analysis. Detailed morphological characterization of C. malayensis was described and illustrated with light and scanning electron microscope images. C. malayensis from Viet Nam had morphological features similar to previous descriptions of the species, including the 3”’ plate being larger than the 4”’ plate. However, the shape of the 3’ plate of C. malayensis from Viet Nam is pentagonal, while it is quadrangular in the original description from Malaysia. In phylogenetic analyses of the LSU rDNA (D8-D10 region), the Vietnamese strains of C. malayensis were grouped with the Macauley Island (New Zealand) strain and the strain of Coolia sp. from Japan with full support value (ML = 100 and BI = 1.0). The intraspecific pairwise distance (p-distance) and the number of different nucleotides among all C. malayensis strains from Viet Nam, New Zealand and Japan ranged from 0 % (0 bp) to 0.12 % (1 bp), and from 0.25 % (2 bp) to 0.37 % (3 bp), respectively.

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References

1. David H., Laza-Martínez A., Rodríguez F., Fraga S., Orive E. – Coolia guanchica sp. nov. (Dinophyceae) a new epibenthic dinoflagellate from the Canary Islands (NE Atlantic Ocean). Eur. J. Phycol., 55 (2019) 76–88. https://doi.org/10.1080/09670262.2019.1651400.

2. Gómez F., Qiu D., Otero-Morales E., Lopes R. M., Lin S. – Circumtropical distribution of the epiphytic dinoflagellate Coolia malayensis (Dinophyceae): morphology and molecular phylogeny from Puerto Rico and Brazil. Phycol. Res., 64 (2016) 194–199. https://doi.org/10.1111/pre.12134.

3. Zhang H., Lü S., Cen J., Li Y., Li Q., Wu Z. – Morphology and molecular phylogeny of three species of Coolia (dinophyceae) from Hainan Island, South China Sea. J. Oceanol. Limnol., 39 (2020) 1020–1032. https://doi.org/10.1007/s00343-020-9326-z.

4. Li X., Yan M., Gu J., et al. – The effect of temperature on physiology, toxicity and toxin content of the benthic dinoflagellate Coolia malayensis from a seasonal tropical region. Water Res., 185 (2020) 116264. https://doi.org/10.1016/j.watres.2020.116264.

5. Meunier A. – Microplancton de la Mer flamande. 4me partie. Les Tintinnides et cetera. Mém. Mus. R. Hist. Nat. Belg., 8 (1919) 59.

6. Faust M. A. – Observation of sand-dwelling toxic dinoflagellates (Dinophyceae) from widely differing sites, including two new species. J. Phycol., 31 (1995) 996–1003. https://doi.org/10.1111/j.0022-3646.1995.00996.x.

7. Ten-Hage L., Turquet J., Quod J. P., Couté A. – Coolia areolata sp. nov. (Dinophyceae), a new sand-dwelling dinoflagellate from the southwestern Indian Ocean. Phycologia, 39 (2000) 377–383. https://doi.org/10.2216/i0031-8884-39-5-377.1.

8. Leaw C.-P., Lim P.-T., Cheng K.-W., Ng B.-K., Usup G. – Morphology and molecular characterization of a new species of thecate benthic dinoflagellate, Coolia malayensis sp. nov. (Dinophyceae). J. Phycol., 46 (2010) 162–171. https://doi.org/10.1111/j.1529-8817.2009.00778.x.

9. Jeong H. J., Yih W., Kang N. S., et al. – First report of the epiphytic benthic dinoflagellates Coolia canariensis and Coolia malayensis in the waters off Jeju Island, Korea: morphology and rDNA sequences. J. Eukaryot. Microbiol., 59 (2012) 114–133. https://doi.org/10.1111/j.1550-7408.2012.00610.x.

10. Karafas S., York R., Tomas C. – Morphological and genetic analysis of the Coolia monotis species complex with the introduction of two new species, Coolia santacroce sp. nov. and Coolia palmyrensis sp. nov. (Dinophyceae). Harmful Algae, 46 (2015) 18–33. https://doi.org/10.1016/j.hal.2015.05.002.

11. Morquecho L., Gárate-Lizárraga I., Gu H. – Morphological and molecular characterization of the genus Coolia (dinophyceae) from bahía de la paz, southwest gulf of california. Algae, 37 (2022) 185–204. https://doi.org/10.4490/algae.2022.37.9.2.

12. Nascimento S. M., da Silva R. A. F., Oliveira F., Fraga S., Salgueiro F. – Morphology and molecular phylogeny of Coolia tropicalis, Coolia malayensis and a new lineage of the Coolia canariensis species complex (Dinophyceae) isolated from Brazil. Eur. J. Phycol., 54 (2019) 484–496. https://doi.org/10.1080/09670262.2019.1599449.

13. Leung P. T. Y., Yan M., Yiu S. K. F., et al. – Molecular phylogeny and toxicity of harmful benthic dinoflagellates Coolia (Ostreopsidaceae, Dinophyceae) in a sub-tropical marine ecosystem: the first record from Hong Kong. Mar. Pollut. Bull., 124 (2017) 878–889. https://doi.org/10.1016/j.marpolbul.2017.01.017.

14. Verma A., Hoppenrath M., Smith K. F., et al. – Ostreopsis schmidt and Coolia meunier (dinophyceae, gonyaulacales) from cook islands and niue (south pacific ocean), including description of Ostreopsis tairoto sp. nov. Sci. Rep., 13 (2023) 3110. https://doi.org/10.1038/s41598-023-29969-z.

15. Abdennadher M., Zouari A. B., Medhioub W., Penna A., Hamza A. – Characterization of Coolia spp. (Gonyaucales, dinophyceae) from southern tunisia: first record of Coolia malayensis in the mediterranean sea. Algae, 36 (2021) 175–193. https://doi.org/10.4490/algae.2021.36.6.2.

16. Tibiriçá C. E. J. de A., Sibat M., Fernandes L. F., et al. – Diversity and toxicity of the genus Coolia Meunier in Brazil, and detection of 44-methyl gambierone in Coolia tropicalis. Toxins, 12 (2020) 327. https://doi.org/10.3390/toxins12050327.

17. Ho V. T., Nguyen N. L., Doan N. H. – Benthic dinoflagellates in Vietnamese waters, Vietnam Academy of Science and Technology, Ha Noi, Viet Nam (2011).

18. Nguyen N. L., Larsen J. – Gonyaulacales. In: Guide to the identification of potentially toxic microalgae in Vietnamese waters, 140, (2004) 73–116.

19. Thuoc C. V. – Data on the species composition and the distribution of harmful marine epiphytic microalgae living on coral reefs in the North of Vietnam. Acad. J. Biol., 24 (2002) 22–30.

20. Ho T. V., Nguyen L. N. – Morphology and distribution of the three epiphytic dinoflagellate species Coolia monotis, C. tropicalis, and C. canariensis (Ostreopsidaceae, Gonyaulacales, Dinophyceae) from Vietnamese coastal waters. Ocean Sci. J., 49 (2014) 211–221. https://doi.org/10.1007/s12601-014-0021-x.

21. Fritz L., Triemer R. E. – A rapid simple technique utilizing Calcofluor White M2R for the visualization of dinoflagellate thecal plates. J. Phycol., 21 (1985) 662–664. https://doi.org/10.1111/j.0022-3646.1985.00662.x.

22. Kofoid C. A. – On Peridinium steinii Jorgensen with a note on the nomenclature of the skeleton of the Peridinidae. In: On Peridinium steinii Jorgensen, with a note on the nomenclature of the skeleton of the Peridinidae and the morphology of the skeleon of Podolampas, 16, (1909) 25–47.

23. Balech E. – Étude des dinoflagellés du sable de Roscoff. Rev. Algol., 2 (1956) 29–52.

24. Chinain M., Faust M. A., Pauillac S. – Morphology and molecular analyses of three toxic species of Gambierdiscus (Dinophyceae): G. pacificus, sp. nov., G. australes, sp. nov., and G. polynesiensis, sp. nov. J. Phycol., 35 (1999) 1282–1296. https://doi.org/10.1046/j.1529-8817.1999.3561282.x.

25. Nishimura S., Manabe I., Takaki S., et al. – Adipose natural regulatory B cells negatively control adipose tissue inflammation. Cell Metab., 18 (2013) 759–766. https://doi.org/10.1016/j.cmet.2013.09.017.

26. Rhodes L., Smith K., Papiol G. G., Adamson J., Harwood T., Munday R. – Epiphytic dinoflagellates in sub-tropical New Zealand, in particular the genus Coolia Meunier. Harmful Algae, 34 (2014) 36–41. https://doi.org/10.1016/j.hal.2014.02.004.

27. Tamura K., Stecher G., Kumar S. – MEGA11: molecular evolutionary genetics analysis version 11. Mol. Biol. Evol., 38 (2021) 3022–3027. https://doi.org/10.1093/molbev/msab120.

28. Darriba D., Taboada G. L., Doallo R., Posada D. – jModelTest 2: more models, new heuristics and parallel computing. Nat. Methods, 9 (2012) 772–772. https://doi.org/10.1038/nmeth.2109.

29. Stamatakis A. – RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics, 30 (2014) 1312–1313. https://doi.org/10.1093/bioinformatics/btu033.

30. Ronquist F., Teslenko M., van der Mark P., et al. – MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst. Biol., 61 (2012) 539–542. https://doi.org/10.1093/sysbio/sys029.

31. Mohammad-Noor N., Moestrup Ø., Lundholm N., et al. – Autecology and phylogeny of Coolia tropicalis and Coolia malayensis (Dinophyceae), with emphasis on taxonomy of C. tropicalis based on light microscopy, scanning electron microscopy and LSU rDNA. J. Phycol., 49 (2013) 536–545. https://doi.org/10.1111/jpy.12062.

32. Tawong W., Nishimura T., Sakanari H., Sato S., Yamaguchi H., Adachi M. – Characterization of Gambierdiscus and Coolia (Dinophyceae) isolates from Thailand based on morphology and phylogeny. Phycol. Res., 63 (2014) 125–133. https://doi.org/10.1111/pre.12074.

33. Wakeman K. C., Yamaguchi A., Roy M. C., Jenke-Kodama H. – Morphology, phylogeny and novel chemical compounds from Coolia malayensis (Dinophyceae) from Okinawa, Japan. Harmful Algae, 44 (2015) 8–19. https://doi.org/10.1016/j.hal.2015.02.009.

34. Larsson M. E., Smith K. F., Doblin M. A. – First description of the environmental niche of the epibenthic dinoflagellate species Coolia palmyrensis, C. malayensis, and C. tropicalis (Dinophyceae) from Eastern Australia. J. Phycol., 55 (2019) 565–577. https://doi.org/10.1111/jpy.12833.

35. Rhodes L. L., Smith K. F., Murray S., Harwood D. T., Trnski T., Munday R. – The epiphytic genus Gambierdiscus (Dinophyceae) in the Kermadec Islands and Zealandia regions of the southwestern Pacific and the associated risk of ciguatera fish poisoning. Mar. Drugs, 15 (2017) 219. https://doi.org/10.3390/md15070219.

36. Hoppenrath M., Murray S. A., Chomérat N., Horiguchi T. – Marine benthic dinoflagellates: unveiling their worldwide biodiversity, Schweizerbart Science Publishers, Stuttgart (2014).

37. Leaw C. P., Tan T. H., Lim H. C., et al. – New scenario for speciation in the benthic dinoflagellate genus Coolia (Dinophyceae). Harmful Algae, 55 (2016) 137–149. https://doi.org/10.1016/j.hal.2016.02.010.

38. Fraga S., Penna A., Bianconi I., Paz B., Zapata M. – Coolia canariensis sp. nov. (Dinophyceae), a new nontoxic epiphytic benthic dinoflagellate from the Canary Islands. J. Phycol., 44 (2008) 1060–1070. https://doi.org/10.1111/j.1529-8817.2008.00555.x.

39. Uribe E., Álvarez G., Cárcamo C., Díaz P. A., Rengel J., Blanco J. – First report of epiphytic dinoflagellate Coolia malayensis (Dinophyceae) in the southeastern Pacific Ocean. J. Phycol., 59 (2023) 725–737. https://doi.org/10.1111/jpy.13351.