Isolation, identification, and evaluation of antimicrobial activity of fungi isolated from marine samples collected at Bach Long Vi Island

Mai Anh Nguyen; Thu Huyen Vu; Hoang Duong Nguyen; Thi Mai Huong Doan; Van Cuong Pham; Thi Hong Minh Le

doi:10.15625/1859-3097/23490

Author affiliations

Authors

Nguyen Mai Anh Institute of Chemistry, VAST, Vietnam
Vu Thu Huyen Institute of Chemistry, VAST, Vietnam
Nguyen Hoang Duong Calidi Biotherapeutics, 4475 Executive Dr Suite 200, San Diego, CA 92121, United States
Doan Thi Mai Huong Institute of Chemistry, VAST, Vietnam
Pham Van Cuong Institute of Chemistry, VAST, Vietnam
Le Thi Hong Minh Institute of Chemistry, VAST, Vietnam

DOI:

https://doi.org/10.15625/1859-3097/23490

Keywords:

Aspergillus, antimicrobial activity, Penicillium malachiteum, marine fungi, marine-derived bioactive extract, MIC

Abstract

This study aimed to isolate and evaluate the antimicrobial properties of fungi collected from Bach Long Vi Island. Seventeen fungal isolates were obtained from fifteen marine samples collected from various locations around the island. The antimicrobial activity of ethyl acetate extracts from these isolates was assessed using the broth microdilution method against seven test microorganisms. The findings indicated that a significant proportion of the isolates demonstrated potent antimicrobial activity, with 82% inhibiting the growth of at least three pathogenic microbes. All fungal isolates exhibited highly variable inhibitory effects against Enterococcus faecalis, with minimum inhibitory concentrations (MIC) ranging from 32 µg to 256 µg. Additionally, eleven of the seventeen fungal strains showed activity against Candida albicans. Notably, strains CL475, CL503, and CL508 exhibited antimicrobial activity against 4-5 target microbial species and were further analyzed for taxonomic identification. The results revealed that strain CL503 and CL508 belong to Aspergillus versicolor, CL508 belongs to Aspergillus nidulans, and strain CL475 was identified as Penicillium malachiteum. Phylogenetic analysis of these promising isolates was conducted using MegaX software. The results of this study suggest that the production and extraction of bioactive compounds by marine fungi may serve as a.

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