6β-hydroxygeniposide, a potent α-glucosidase inhibitor from <i>Gardenia jasminoides</i> growing in Viet Nam

Vu Dinh Hoang; Nguyen Khac Hung; Pham Quang Duong; Nguyen Thi Viet Thanh; Dinh Thi Phuong Anh; Chu Van Tan; Cao Duc Tuan; Tran Hong Ngoc; Truong Gia Bao; Tran Dai Lam

doi:10.15625/2525-2518/20030

Author affiliations

Authors

Vu Dinh Hoang \(^1\) Hanoi University of Science and Technology, 1 Dai Co Viet street, Bach Mai ward, Ha Noi, Viet Nam https://orcid.org/0000-0002-2112-6732
Nguyen Khac Hung \(^1\) Hanoi University of Science and Technology, 1 Dai Co Viet street, Bach Mai ward, Ha Noi, Viet Nam https://orcid.org/0000-0001-5972-0131
Pham Quang Duong \(^2\) Institute of Materials Science, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet street, Nghia Do ward, Ha Noi, Viet Nam
Nguyen Thi Viet Thanh \(^1\) Hanoi University of Science and Technology, 1 Dai Co Viet street, Bach Mai ward, Ha Noi, Viet Nam
Dinh Thi Phuong Anh \(^1\) Hanoi University of Science and Technology, 1 Dai Co Viet street, Bach Mai ward, Ha Noi, Viet Nam
Chu Van Tan \(^3\) High Technology Innovation Center, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet street, Nghia Do ward, Ha Noi, Viet Nam
Cao Duc Tuan \(^4\) Hai Phong University of Medicine and Pharmacy, 72A Nguyen Binh Khiem street, Gia Vien ward, Hai Phong, Viet Nam https://orcid.org/0000-0001-9129-8241
Tran Hong Ngoc \(^5\) Faculty of Pharmacy, Phenikaa University, Duong Noi ward, Ha Noi, Viet Nam
Truong Gia Bao \(^6\) Gifted Class for Chemistry, High School for Gifted Students, Hanoi University of Science, Vietnam National University, Ha Noi, Viet Nam
Tran Dai Lam \(^2\) Institute of Materials Science, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet street, Nghia Do ward, Ha Noi, Viet Nam

DOI:

https://doi.org/10.15625/2525-2518/20030

Keywords:

6β-hydroxygeniposide, anti alpha-glucosidase, diabetes, Gardenia jasminoides, molecular docking, molecular dynamic simulation

Abstract

According to the statistics of WHO in 2019, diabetes was one of the top 10 causes of death in Viet Nam and the incidence increasing day by day has required more effective treatment methods. For a long time, natural products have played an important role in drug screening programs. Gardenia jasminoides belongs to the Gardenia genus of the Rubiaceae family, in which previous research showed potential ability in diabetes treatment through various mechanisms. In this study, 3 iridoids were isolated from G. jasminoides collected in Viet Nam and the a-glucosidase inhibitory activity was evaluated for the first time. The results showed that 6b-hydroxygeniposide (2) expressed the strongest activity with IC₅₀ at 6.38 ± 0.12 mM, while geniposide (1) and 6a-hydroxygeniposide (3) were inactive. The inhibitory mechanism of 6b- hydroxygeniposide was revealed by molecular docking and molecular dynamics. 6b- Hydroxygeniposide competed and blocked p-nitrophenyl-a-D-glucopyranoside substrate reaching the reaction center of the enzyme, which was expressed through lower non-bonding interaction energy and stable binding affinity.

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