A study on in vitro propagation of Polygonatum kingianum Coll. et Hemsl.
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https://doi.org/10.15625/2615-9023/23056Keywords:
Callus, humic, medicinal, Polygonatum kingianum, propagationAbstract
Polygonatum kingianum is a valuable medicinal species widely utilized in traditional medicine owing to its rich nutritional and antioxidant properties. However, natural populations are rapidly declining due to overexploitation and inherently low reproductive capacity. This study aimed to develop an efficient in vitro propagation protocol using young leaf and rhizome explants. The optimal surface sterilization was achieved using 0.1% HgCl2 for 6 minutes (leaf) and 12 minutes (rhizome), resulting in survival rates of 76.67% and 53.33%, respectively. Callus induction was most effective on MS medium supplemented with 1.0 mg/L TDZ and 0.5 mg/L 2,4-D, with an induction rate of 83.33% and fresh weight of 0.943 g. Shoot regeneration was optimized on MS medium containing 1.5 mg/L TDZ and 0.2 mg/L NAA, producing an average of 13.67 shoots per explant with a mean shoot length of 5.02 cm. Rooting was best achieved on half-strength MS medium supplemented with 1.0 mg/L NAA, yielding a 100% rooting rate, 4.9 roots per shoot, and 2.93 cm average root length. The inclusion of humic acid significantly enhanced root induction, with 1.5 mg/L being the most effective concentration. Acclimatization trials revealed a 100% survival rate in plantlets grown in a substrate mixture of coconut fiber powder, fern fiber, and compost (6:3:1), which also promoted strong root development. This is the first report demonstrating the positive effect of humic acid on root induction in P. kingianum, offering a promising approach for large-scale propagation and conservation.
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Copyright (c) 2026 Dang Thi Tham, Nguyen Thi Thanh Hang, Tran Thai Vinh, Quach Van Hoi, Vu Kim Cong, Nong Van Duy
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