Study on culture conditions to enhance the production of α- glucosidase inhibitor, acarbose, from <i>Actinoplanes hulinensis</i> 1094

Thi Trung Nguyen; Nhat Linh Nguyen; Minh Duc Luu; Thanh Hoang Le; Thi Anh Tuyet Nguyen; Thanh Tuan Tran; Duy Nam Pham; Thi Tuyen Do

doi:10.15625/vjbt-22442

Author affiliations

Authors

Thi Trung Nguyen \(^1\) Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi, Vietnam
Nhat Linh Nguyen \(^2\) Institute of Biology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi, Vietnam
Minh Duc Luu \(^2\) Institute of Biology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi, Vietnam
Thanh Hoang Le \(^2\) Institute of Biology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi, Vietnam
Thi Anh Tuyet Nguyen \(^2\) Institute of Biology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi, Vietnam
Thanh Tuan Tran \(^1\) Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi, Vietnam
\(^3\) Vietnam-Russia tropical center, 63 Nguyen Van Huyen, Nghia Do, Hanoi, Vietnam
Duy Nam Pham \(^3\) Vietnam-Russia tropical center, 63 Nguyen Van Huyen, Nghia Do, Hanoi, Vietnam
Thi Tuyen Do \(^1\) Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi, Vietnam
\(^2\) Institute of Biology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi, Vietnam https://orcid.org/0000-0002-8048-5794

DOI:

https://doi.org/10.15625/vjbt-22442

Keywords:

Acarbose,, Actinoplanes hulinensis 1094,, αglucosidase,, fermentation optimization,, type 2 diabetes mellitus.

Abstract

Alpha-glucosidase inhibitors are a class of drugs that are widely used in the treatment of type 2 diabetes mellitus. Members of this class such as acarbose, is a pseudo-oligosaccharide that acts as a competitive α-glucosidase inhibitor and has been widely used in the treatment of type 2 diabetes due to its ability to regulate postprandial blood glucose levels. they competitively inhibit enzymes that convert complex non-absorbable carbohydrates into simple absorbable carbohydrates by delaying carbohydrate absorption, they reduce the rise in postprandial blood glucose. Acarbose has been shown to increase life expectancy in patients with type 2 diabetes mellitus and reduces the risk of the development of cardiovascular events in patients with impaired glucose tolerance. In this study, we aimed to enhance the biosynthesis of acarbose by optimizing the fermentation conditions of Actinoplanes hulinensis 1094. In 7 surveyed media (MT1, MT2, MT3, MT4, MT5, MT6, and MT7), the α-glucosidase inhibitory activity was highest in MT7 medium, the activity was 58.6%, the lowest was in MT6 medium. Component optimization of MT7 led to the formulation of an optimal medium containing (g/L): 50 maltose, 15 corn flour, 1 monosodium glutamate, 2 CaCl₂, 0.5 FeCl₃, 1.0 K₂HPO₄, and 2.5 CaCO₃. Subsequent optimization of physical conditions identified 30°C, pH 7.0, and 200 rpm as the most favorable parameters. Under these optimized conditions, the α-glucosidase inhibitory activity reached 82.2%, surpassing that of the 1 mg/mL standard acarbose control. These findings highlight the potential of A. hulinensis 1094 as a promising strain for high-yield acarbose production under cost-effective and scalable conditions.

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