The proliferation of hematopoietic stem cells from umbilical cord blood was enhanced by using peptide sl-13r

Vu Thi Tien; Tran Trung Thanh; Tran Trung Kien; Dinh Ho Thao An; Nguyen Trung Nam

doi:10.15625/vjbt-20996

Author affiliations

Authors

Vu Thi Tien \(^1\) Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam https://orcid.org/0009-0008-5450-4702
Tran Trung Thanh \(^2\) Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam https://orcid.org/0000-0001-6851-636X
Tran Trung Kien \(^3\) Hanoi Obstetrics and Gynecology Hospital, 929 La Thanh Road, Ba Dinh District, Hanoi, Vietnam
Dinh Ho Thao An \(^4\) University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
Nguyen Trung Nam \(^1\) Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
\(^2\) Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam

DOI:

https://doi.org/10.15625/vjbt-20996

Keywords:

Umbilical cord blood, mononuclear cells, hematopoietic stem cells, SL-13R peptide, cell proliferation

Abstract

Umbilical cord hematopoietic stem cells (UC-HSCs) are capable of self-regeneration and differentiation into many different types of blood cells, helping to treat blood diseases such as leukemia, Hodgkin's disease and non-Hodgkin's lymphoma, as well as for transplantation. However, the number of UC-HSCs is still very small due to limitations in isolation and proliferation. In this study, UC-HSCs were cultured with the bioactive SL-13R peptide to examine whether SL-13R enhances UC-HSC proliferation. Here, we showed that CD34⁺UC-HSCs can be enriched by up to 47.08% by culturing total mononuclear cells (MNCs) from umbilical cord blood (UCB) in serum-free StemMACS medium supplemented with a cytokine cocktail. We demonstrated that SL-13R enhanced the proliferation of CD34⁺UC-HSCs by 1.21-1.44 times compared to that of the controls. These results suggested that StemMACS medium supplemented with cytokines and SL-13R can be used to expand CD34⁺UC-HSCs ex vivo for medical treatment and transplantation.

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