Effect of temperature and light intensity on the growth of <em>Eucheuma denticulatum</em> seaweed branches from Van Phong Bay under laboratory conditions

Thi Mo Vu; Trong Nghia Le; Mai Duc Tran; Truong Trung Lien Le; Son Lam Ho; Anuraj Anirudhan

doi:10.15625/1859-3097/22471

Author affiliations

Authors

Vu Thi Mo Institute of Oceanography, VAST, Vietnam https://orcid.org/0009-0005-8765-7201
Le Trong Nhia Institute of Oceanography, VAST, Vietnam https://orcid.org/0009-0003-2977-0834
Tran Mai Duc Institute of Oceanography, VAST, Vietnam https://orcid.org/0000-0001-5652-7133
Le Truong Trung Lien Institute of Oceanography, VAST, Vietnam https://orcid.org/0009-0003-6737-8454
Ho Son Lam Institute of Oceanography, VAST, Vietnam https://orcid.org/0009-0001-2618-8203
Anuraj Anirudhan Mariculture Division, ICAR - Central Marine Fisheries Research Institute, Kochi, India

DOI:

https://doi.org/10.15625/1859-3097/22471

Keywords:

Acclimatization, Eucheuma denticulatum, light intensities, temperature

Abstract

Establishing an initial source of tissue culture material is the first and most important step in the propagation process. To prepare for this stage, seaweed must be acclimatized in vitro for 4 to 8 weeks before sterilization. In this study, Eucheuma denticulatum seaweed branches were collected from seaweed farms operated by local households in Van Phong Bay, Dai Lanh commune, Khanh Hoa province, brought to the laboratory, and acclimatized in a controlled environment. Healthy branches with a glossy brown color were carefully selected, cut into 10–15 cm lengths, and fresh-weight 13–15 grams samples were tied to mesh frames and placed in glass tanks measuring 35 × 40 × 60 cm. Environmental factors such as temperature (22–30^oC) and light intensity (40–200 µmol photons·m^-2·s^-1) were tested. The results showed that after six weeks of acclimation, E. denticulatum branches thrived best at 26^oC, achieving a high survival rate of 73.33%, a fresh weight of 21.38 g, the highest cumulative growth rate of 1.12 %·day^-1, and the lowest ice-ice disease prevalence at 1.11%. Similarly, after six weeks of acclimation, the branches showed optimal growth under specific light intensity conditions of 120 µmol photon·m^-2·s^-1, with a high survival rate of 77.78%, a fresh weight of 28.82 g, the highest cumulative growth rate of 1.77 %·day^-1, and no occurrence of ice-ice disease. The E. denticulatum branches cultivated under optimal conditions are poised to serve as ideal raw materials for propagation through tissue culture techniques, paving the way for efficient production and the replacement of natural seaweed stocks.

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References

[1] G. C. Trono, Jr., “Eucheuma and Kappaphycus: Taxonomy and cultivation,” Bulletin of Marine Sciences and Fisheries, Kochi University, vol. 12, pp. 51–65, 1992.

[2] J. N. Magallanes, R. D. Lauzon, and I. C. Emnace, “Inhibitory potential of Eucheuma denticulatum (N. L. Burman) F. S. Collins & Hervey against selected foodborne pathogens,” Philippine Journal of Science, vol. 150, no. 2, pp. 371–376, 2021.

[3] A. B. Tahiluddin, E. S. Imbuk, J. H. Sarri, H. S. Mohammad, F. N. T. Ensano, M. M. Maddan, and B. S. Cabilin, “Eucheumatoid seaweed farming in the southern Philippines,” Aquatic Botany, vol. 189, 103697, 2023.

[4] Food and Agriculture Organization of the United Nations (FAO), World Fisheries and Aquaculture in Review. Rome, Italy: FAO, 2024.

[5] Q. N. Nguyen, T. M. A. Nguyen, M. H. Vu, and D. T. Dam, “Species diversity and distribution of the macro algae from Bach Long Vi Island, Vietnam,” Vietnam Journal of Marine Science and Technology, vol. 24, no. 4, pp. 363–374, 2024.

[6] T. D. N. Ngo, T. H. T. Phan, E. A. Yurchenko, A. N. Yurchenko, T. T. H. Cao, T. D. T. Vo, T. T. V. Tran, D. T. Pham, H. N. K. Huynh, and T. N. P. Bui, “Antioxidant and cytoprotective activities of marine fungi isolated from brown seaweeds in Nha Trang Bay, Khanh Hoa Province, Central Vietnam,” Vietnam Journal of Marine Science and Technology, vol. 24, no. 3, pp. 297–306, 2024.

[7] V. M. Tuan, N. V. Thanh, and T. D. Lai, “Assessing impacts of climate change-driven sea level rise on hydrodynamics and sediment dynamics along Ba Lang beaches in Nha Trang Bay, Khanh Hoa, Vietnam,” Water Science and Engineering, vol. 17, no. 4, pp. 323–335, 2024.

[8] H. J. Bixler and H. Porse, “A decade of change in the seaweed hydrocolloids industry,” Journal of Applied Phycology, vol. 23, no. 3, pp. 321–335, 2011.

[9] C. S. Vairappan, R. Razalie, U. M. Elias, and T. Ramachandram, “Effects of improved post-harvest handling on the chemical constituents and quality of carrageenan in red alga, Kappaphycus alvarezii Doty,” Journal of Applied Phycology, vol. 26, no. 2, pp. 909–916, 2014.

[10] H. D. Nguyen, D. K. Dang, Y. N. Nguyen, C. P. Van, Q. H. Truong, Q. T. Bui, and A. I. Petrisor, “A framework for flood depth using hydrodynamic modeling and machine learning in the coastal province of Vietnam,” Vietnam Journal of Earth Sciences, vol. 45, no. 4, pp. 456–478, 2023.

[11] H. N. Duc, G. N. Tien, X. H. Le, T. V. Ngoc, and D. N. Huu, “Multi-step-ahead prediction of water levels using machine learning: A comparative analysis in the Vietnamese Mekong Delta,” Vietnam Journal of Earth Sciences, vol. 46, no. 4, pp. 468–488, 2024.

[12] T. L. Giang, K. B. Dang, and Q. T. Bui, “Coastline and shoreline change assessment in sandy coasts based on machine learning models and high-resolution satellite images,” Vietnam Journal of Earth Sciences, vol. 45, no. 2, pp. 251–270, 2023.

[13] W. T. L. Yong, J. Y. Y. Chin, V. Y. Thien, and S. Yasir, “Heavy metal accumulation in field cultured and tissue cultured Kappaphycus alvarezii and Gracilaria changii,” International Food Research Journal, vol. 24, no. 3, pp. 970–975, 2017.

[14] C. R. K. Reddy, G. R. K. Kumar, A. K. Siddhanta, A. Tewari, and K. Eswaran, “In vitro somatic embryogenesis and regeneration of somatic embryos from pigmented callus of Kappaphycus alvarezii (Doty) Doty (Rhodophyta, Gigartinales)1,” Journal of Phycology, vol. 39, no. 3, pp. 610–616, 2003.

[15] A. Azizi, N. Mohd Hanafi, M. N. Basiran, and C. H. Teo, “Evaluation of disease resistance and tolerance to elevated temperature stress of the selected tissue-cultured Kappaphycus alvarezii Doty 1985 under optimized laboratory conditions,” 3 Biotech, vol. 8, no. 8, 321, 2018.

[16] Y. S. Yong, W. T. L. Yong, V. Y. Thien, S. E. Ng, A. Anton, and S. Yassir, “Acclimatization of micropropagated Kappaphycus alvarezii (Doty) Doty ex Silva (Rhodophyta, Solieriaceae) in outdoor nursery system,” Journal of Applied Phycology, vol. 27, no. 1, pp. 413–419, 2015.

[17] W. T. L. Yong, J. Y. Y. Chin, V. Y. Thien, and S. Yasir, “Evaluation of growth rate and semi-refined carrageenan properties of tissue-cultured Kappaphycus alvarezii (Rhodophyta, Gigartinales),” Phycological Research, vol. 62, no. 4, pp. 316–321, 2014.

[18] T. M. Vu, K. S. Nguyen, T. N. Le, V. H. Tran, M. D. Tran, T. T. Vo, N. U. M. Hoang, T. P. Nguyen, and S. L. Ho, “Effects of IAA and KIN on somatic embryogenesis and the effect of Acadian marine plant extract powder content on micropropagule regeneration of the seaweed Kappaphycus striatus,” Vietnam Journal of Marine Science and Technology, vol. 26, no. 1, pp. 45–58, 2026.

[19] M. Ohno, H. Q. Nang, and S. Hirase, “Cultivation and carrageenan yield and quality of Kappaphycus alvarezii in the waters of Vietnam,” Journal of Applied Phycology, vol. 8, no. 4, pp. 431–437, 1996.

[20] Lideman, G. N. Nishihara, T. Noro, and R. Terada, “Effect of temperature and light on the photosynthesis as measured by chlorophyll fluorescence of cultured Eucheuma denticulatum and Kappaphycus sp. (Sumba strain) from Indonesia,” Journal of Applied Phycology, vol. 25, no. 2, pp. 399–406, 2013.

[21] V. T. Mo, Biology, Ecology and Propagation of Kappaphycus striatus, Ph.D. dissertation, Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi, Vietnam, 2022, 167 pp. [in Vietnamese].

[22] S. Suto, “Skeletonema no tame no jinkou baiyoueki,” Suisan Zoushoku, vol. 7, pp. 17–19, 1959. [in Japanese].

[23] G. M. Ward, C. S. Kambey, J. P. Faisan, Jr., P. L. Tan, C. C. Daumich, I. Matoju, G. D. Stentiford, D. Bass, P.-E. Lim, J. Brodie, and S. W. Poong, “Ice-Ice disease: An environmentally and microbiologically driven syndrome in tropical seaweed aquaculture,” Reviews in Aquaculture, vol. 14, no. 1, pp. 414–439, 2022.

[24] C. A. Penniman, A. C. Mathieson, and C. E. Penniman, “Reproductive phenology and growth of Gracilaria tikvahiae McLachlan (Gigartinales, Rhodophyta) in the Great Bay estuary, New Hampshire,” Botanica Marina, vol. 29, no. 2, pp. 147–154, 1986.

[25] P. A. Wenno, R. Syamsuddin, E. N. Zainuddin, and R. Ambo-Rappe, “Cultivation of red seaweed Kappaphycus alvarezii (Doty) at different depths in South Sulawesi, Indonesia,” Aquaculture, Aquarium, Conservation & Legislation, vol. 8, no. 3, pp. 468–473, 2015.

[26] M. Ohno, D. B. Largo, and T. Ikumoto, “Growth rate, carrageenan yield and gel properties of cultured kappa-carrageenan producing red alga Kappaphycus alvarezii (Doty) Doty in the subtropical waters of Shikoku, Japan,” Journal of Applied Phycology, vol. 6, no. 1, pp. 1–5, 1994.

[27] R. D. Villanueva, J. B. Romero, M. N. E. Montaño, and P. O. de la Peña, “Harvest optimization of four Kappaphycus species from the Philippines,” Biomass and Bioenergy, vol. 35, no. 3, pp. 1311–1316, 2011.

[28] J. Mollion and J. P. Braud, “A Eucheuma (Solieriaceae, Rhodophyta) cultivation test on the south-west coast of Madagascar,” Hydrobiologia, vol. 260, no. 1, pp. 373–378, 1993.

[29] S. N. S. Muhamad, A. P. K. Ling, and C. L. Wong, “Effect of plant growth regulators on direct regeneration and callus induction from Sargassum polycystum C. Agardh,” Journal of Applied Phycology, vol. 30, no. 6, pp. 3299–3310, 2018.

[30] M. Jum’at, N. Cokrowati, and S. Y. Lumbessy, “Cultivation of seaweed Kappaphycus alvarezii with various substrates different on laboratory scale,” Journal of Coastal and Ocean Sciences, vol. 5, no. 1, pp. 53–61, 2024.

[31] A. Arasamuthu and J. P. Edward, “Occurrence of ice-ice disease in seaweed Kappaphycus alvarezii at Gulf of Mannar and Palk Bay, southeastern India,” Indian Journal of Geo-Marine Sciences, vol. 47, no. 6, pp. 1208–1216, 2018.

[32] G. Tsiresy, J. Preux, T. Lavitra, P. Dubois, G. Lepoint, and I. Eeckhaut, “Phenology of farmed seaweed Kappaphycus alvarezii infestation by the parasitic epiphyte Polysiphonia sp. in Madagascar,” Journal of Applied Phycology, vol. 28, no. 5, pp. 2903–2914, 2016.

[33] F. E. Msuya, “Seaweed farming as a potential cluster,” in Proc. Innovation Systems and Clusters Programme in Tanzania (ISCP-Tz): Cluster Initiative Launching Workshop, B. L. M. Mwamila and A. K. Temu, Eds., Dar es Salaam, Tanzania, Feb. 2006, pp. 102–113.