Effects of IAA and kin on somatic embryogenesis and the effect of acadian marine plant extract powder content on micropropagule regeneration of the seaweed <i>Kappaphycus striatus</i>

Thi Mo Vu; Ky Sanh Nguyen; Trong Nghia Le; Van Huynh Tran; Mai Duc Tran; Thanh Trung Vo; Nhat Uyen My Hoang; Thuy Phuong Nguyen; Son Lam Ho

doi:10.15625/1859-3097/23495

Author affiliations

Authors

Vu Thi Mo Institute of Oceanography, VAST, Vietnam
Nguyen Ky Sanh VINA Biological seafoods joint stock company, Khanh Hoa, Vietnam
Le Trong Nghia Institute of Oceanography, VAST, Vietnam
Tran Van Huynh Institute of Oceanography, VAST, Vietnam
Tran Mai Duc Institute of Oceanography, VAST, Vietnam
Vo Thanh Trung Institute of Oceanography, VAST, Vietnam
Hoang Nhat Uyen My Nha Trang University, Khanh Hoa, Vietnam
Nguyen Thuy Phuong Institute of Oceanography, VAST, Vietnam
Ho Son Lam Institute of Oceanography, VAST, Vietnam

DOI:

https://doi.org/10.15625/1859-3097/23495

Keywords:

AMPEP, callus, IAA, KIN, Kappaphycus striatus, somatic embryos

Abstract

The objective of this study was to perfect the callus culture method for propagating Kappaphycus striatus. Our research focuses on enhancing somatic embryo (SE) induction from callus and on micropropagule regeneration in SEs. To enhance the induction rate of SEs, the 16-week-old callus was transplanted into a semi-solidified PES (0.4% agar) medium supplemented with 0.5–3.0 mg.L^-1 3-indoleacetic acid (IAA) and 0.5–3.0 mg.L^-1 kinetin (KIN), either alone or in combination. The results showed that transplanting the callus into a PES medium containing IAA and KIN (0.5–3.0 mg L^-1), either individually or in combination, significantly affected somatic embryogenesis in K. striatus. After 8 weeks of cultivation, the treatment with 1 mg.L^-1 IAA + 2 mg.L^-1 KIN exhibited the highest embryo induction rate (62.22 ± 5.09%) and the greatest number of SEs (182.00 ± 10.58 embryos/explant) compared to other treatments. Moreover, during the micropropagule regeneration stage, the commercial powdered extract of Ascophyllum nodosum (Acadian Marine Plant Extract Powder, AMPEP) at concentrations ranging from 5 ppm to 50 ppm was found to enhance the rate of micropropagule regeneration from SEs of K. striatus. The optimal regeneration of SEs of K. striatus into complete micropropagules occurred in an AMPEP medium at a concentration of 20 ppm. Micropropagules cultured in this medium displayed the highest survival rate (75.6 ± 5.09%), number of branches (7.67 ± 0.58 branches/micropropagule), branch length (24.7 ± 1.53; 25 ± 1.00 mm), and fresh weight (173.7 ± 8.14 mg) compared to alternative treatments.

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References

[1] P. I. Hargreaves, C. A. Barcelos, A. C. A. da Costa, and N. Pereira Jr., “Production of ethanol 3G from Kappaphycus alvarezii: Evaluation of different process strategies,” Bioresource Technology, vol. 134, pp. 257–263, 2013.

[2] M. D. Meinita, Y. K. Hong, and G. T. Jeong, “Detoxification of acidic catalyzed hydrolysate of Kappaphycus alvarezii (cottonii),” Bioprocess and Biosystems Engineering, vol. 35, no. 1–2, pp. 93–98, 2012.

[3] N. Q. Nga, N. Thi, M. Anh, V. M. Hung, and D. D. Tien, “Species diversity and distribution of macroalgae from Bach Long Vi Island,” Vietnam Journal of Marine Science and Technology, vol. 24, no. 4, pp. 363–374, 2024.

[4] N. Thi, T. Hang, N. Van Tu, V. Van Phu, and N. N. Lam, “Species composition and distribution of seaweeds in Phu Yen province,” Vietnam Journal of Marine Science and Technology, vol. 20, no. 4, pp. 69–80, 2020.

[5] N. D. Nghia, P. T. M. Thu, “Asparagopsis taxiformis: Potential and perspective for Vietnam seaweed industry to reduce CH₄ emission in animal husbandry,” Vietnam Journal of Marine Science and Technology, vol. 25, no. 3, pp. 373–386, 2025.

[6] D. C. Thung, “Research results of biological resources and biodiversity of Vietnam sea: Current status, threats, and proposed solutions for sustainable use,” Vietnam Journal of Marine Science and Technology, vol. 22, no. 1, pp. 1–20, 2022.

[7] R. Adharini, E. Suyono, S. Suadi, A. Jayanti, and A. Setyawan, “A comparison of nutritional values of Kappaphycus alvarezii, Kappaphycus striatum, and Kappaphycus spinosum from the farming sites in Gorontalo Province, Sulawesi, Indonesia,” Journal of Applied Phycology, vol. 31, no. 1, pp. 725–730, 2018.

[8] L. Hayashi and R. P. Reis, “Cultivation of the red algae Kappaphycus alvarezii in Brazil and its pharmacological potential,” Brazilian Journal of Pharmacognosy, vol. 22, no. 4, pp. 748–752, 2012.

[9] M. L. Wells, P. Potin, J. S. Craigie, J. A. Raven, S. S. Merchant, K. E. Helliwell, A. G. Smith, and M. E. Camire, “Algae as nutritional and functional food sources: Revisiting our understanding,” Journal of Applied Phycology, vol. 29, no. 2, pp. 949–982, 2017.

[10] V. T. Mo, L. K. Cuong, H. T. Tung, T. V. Huynh, L. N. Nghia, C. M. Khanh, N. N. Lam, and D. T. Nhut, “Somatic embryogenesis and plantlets regeneration from seaweed Kappaphycus striatus,” Acta Physiologiae Plantarum, vol. 42, pp. 104–115, 2020.

[11] C. R. K. Reddy, S. A. K. Kumar, A. Tewari, and K. Eswaran, “In vitro somatic embryogenesis and regeneration of somatic embryos from pigmented callus of Kappaphycus alvarezii (Doty) Doty (Rhodophyta, Gigartinales),” Journal of Phycology, vol. 39, no. 1, pp. 610–616, 2003.

[12] N. S. Yokoya, J. A. West, and L. A. E. West, “Effects of plant growth regulators on callus formation, growth and regeneration in axenic tissue cultures of Gracilaria tenuistipitata and Gracilaria perplexa (Gracilariales, Rhodophyta),” Phycological Research, vol. 52, no. 3, pp. 244–254, 2004.

[13] P. Baweja and D. Sahoo, “Regeneration studies in Grateloupia filicina (J. V. Lamouroux) C. Agardh: An important carrageenophyte and edible seaweed,” Algae, vol. 24, no. 3, pp. 163–168, 2009.

[14] P. Baweja, D. Sahoo, P. García-Jiménez, and R. R. Robaina, “Seaweed tissue culture as applied to biotechnology: Problems, achievements and prospects,” Phycological Research, vol. 57, no. 1, pp. 45–58, 2009.

[15] E. R. Tarakhovskaya, Y. I. Maslov, and M. F. Shishova, “Phytohormones in algae,” Russian Journal of Plant Physiology, vol. 54, no. 2, pp. 163–170, 2007.

[16] W. A. Stirk, D. Tarkowská, V. Turečová, and M. Strnad, “Abscisic acid, gibberellins and cytokinins in algae,” Journal of Applied Phycology, vol. 26, no. 1, pp. 561–567, 2014.

[17] G. Garcia-Reina, R. R. Romero, and A. Luque, “Regeneration of thalli clones from Laurencia sp. (Rhodophyta),” Plant Cell Biotechnology, vol. 18, pp. 81–86, 1988.

[18] W. M. Elakbawy, S. M. M. Shanab, and E. A. Shalaby, “Enhancement of plant growth regulators production from microalgae cultivated in treated sewage wastewater (TSW),” BMC Plant Biology, vol. 22, no. 377, pp. 1–11, 2022.

[19] Z. Li, Y. Shi, M. Zhao, X. Zhang, Y. Liu, and Q. Han, “Plant growth regulators improve the growth and physiology of transplanted Thalassia hemprichii fragments,” Frontiers in Marine Science, vol. 11, article 1334937, pp. 1–13, 2024.

[20] A. Fehér, “Callus, dedifferentiation, totipotency, and somatic embryogenesis: What these terms mean in the era of molecular plant biology?,” Frontiers in Plant Science, vol. 10, article 536, pp. 1–11, 2019.

[21] Z. Sung and R. Okimoto, “Embryonic proteins in somatic embryos of carrot,” Proceedings of the National Academy of Sciences of the United States of America, vol. 78, no. 6, pp. 3683–3687, 1981.

[22] W. A. Stirk and J. van Staden, “Heavy metal detoxification in macroalgae: Physiological and biochemical mechanisms,” Phycologia, vol. 53, no. 5, pp. 445–456, 2014.

[23] A. Q. Hurtado, I. C. Neish, and A. T. Critchley, “Developments in production technology of Kappaphycus in the Philippines: More than four decades of farming,” Journal of Applied Phycology, vol. 27, no. 5, pp. 1945–1961, 2015.

[24] R. G. Marroig, R. R. Loureiro, and R. P. Reis, “The effect of Ascophyllum nodosum (Ochrophyta) extract powder on the epibiosis of Kappaphycus alvarezii (Rhodophyta) commercially cultivated on floating rafts,” Journal of Applied Phycology, vol. 28, no. 4, pp. 2471–2477, 2016.

[25] R. R. Loureiro, R. P. Reis, and R. G. Marroig, “Effect of the commercial extract of the brown alga Ascophyllum nodosum Mont. on Kappaphycus alvarezii (Doty) Doty ex P. C. Silva in situ submitted to lethal temperatures,” Journal of Applied Phycology, vol. 26, no. 1, pp. 629–634, 2014.

[26] J. S. Craigie, “Seaweed extract stimuli in plant science and agriculture,” Journal of Applied Phycology, vol. 23, no. 3, pp. 371–393, 2011.

[27] M. K. M. Ali, S. M. Yasir, A. T. Critchley, and A. Q. Hurtado, “Impacts of Ascophyllum marine plant extract powder (AMPEP) on the growth, incidence of the endophyte Neosiphonia apiculata and associated carrageenan quality of three commercial cultivars of Kappaphycus in Sabah, Malaysia,” Journal of Applied Phycology, vol. 30, no. 2, pp. 1185–1195, 2018.

[28] H. A. I. Hadjiran and A. Illud, “Effects of organic fertilizers on the growth performance, ‘Ice-Ice’ disease occurrence and carrageenan quality of farmed seaweed Kappaphycus striatus (F. Schmitz) Doty ex P. C. Silva,” International Journal of Mechanical and Production Engineering Research and Development, vol. 10, no. 3, pp. 12313–12330, 2020.

[29] D. B. Duncan, “Multiple range and multiple F tests,” Biometrics, vol. 11, no. 1, pp. 1–42, 1995.

[30] D. T. Nhut, Plant Biotechnology. The Agricultural Publishing House, 2011, pp. 1–531. [in Vietnamese].

[31] P. T. Mat, D. D. Thu, and N. V. Nguyen, “Study on breeding seaweed (Kappaphycus alvarezii) using tissue culture method,” Journal of Agriculture and Rural Development, no. 12, pp. 124–131, 2019. [in Vietnamese].

[32] J. Arditti, “Micropropagation of orchids: A review on the potential of different explants,” Scientia Horticulturae, vol. 122, no. 4, pp. 507–520, 2009.

[33] J. T. Yoon and W. Y. Soh, “Developmental morphology on the regeneration of Pelvetia siliquosa Tseng et Chang (Phaeophyta) in Korea,” Algae, vol. 13, no. 2, pp. 261–270, 1998.

[34] E. Sulistiani and D. T. Soelistyowati, “Callus induction and filament regeneration from callus of cottonii seaweed (Kappaphycus alvarezii (Doty)) collected from Natuna Islands, Riau Islands Province,” Biotropia, vol. 19, pp. 103–114, 2015.

[35] N. S. Yokoya, M. Avila, M. I. Piel, F. Villanueva, and A. Alcapan, “Effects of plant growth regulators on growth and morphogenesis in tissue culture of Chondracanthus chamissoi (Gigartinales, Rhodophyta),” Journal of Applied Phycology, vol. 26, no. 2, pp. 819–823, 2014.

[36] K. Nitish and M. P. Reddy, “In vitro plant propagation,” Journal of Forest and Environmental Science, vol. 27, no. 2, pp. 61–72, 2011.

[37] P. S. Kakade, S. B. Zimare, and N. P. Malpathak, “Effects of Sargassum ilicifolium seaweed extract on enhanced in vitro seed germination, mass propagation, and accumulation of plumbagin in Plumbago zeylanica L.,” Plant Cell, Tissue and Organ Culture, vol. 149, no. 1–2, pp. 399–410, 2022.

[38] A. Q. Hurtado, D. A. Yunque, K. Tibubos, and A. T. Critchley, “Use of Acadian marine plant extract powder from Ascophyllum nodosum in tissue culture of Kappaphycus varieties,” Journal of Applied Phycology, vol. 21, no. 6, pp. 633–639, 2009.

[39] A. Q. Hurtado, M. Joe, R. C. Sanares, D. Fan, B. Prithiviraj, and A. T. Critchley, “Investigation of the application of Acadian marine plant extract powder (AMPEP) to enhance the growth, phenolic content, free radical scavenging, and iron chelating activities of Kappaphycus Doty (Solieriaceae, Gigartinales, Rhodophyta),” Journal of Applied Phycology, vol. 24, no. 3, pp. 601–611, 2012.

[40] R. R. Loureiro, R. P. Reis, F. D. Berrogain, and A. T. Critchley, “Extract powder from the brown alga Ascophyllum nodosum (Linnaeus) Le Jolis (AMPEP): A ‘vaccine-like’ effect on Kappaphycus alvarezii (Doty) Doty ex P. C. Silva,” Journal of Applied Phycology, vol. 24, no. 3, pp. 427–432, 2012.

[41] C. R. K. Reddy, N. S. Yokoya, W. T. L. Yong, M. R. J. Luhan, and A. Q. Hurtado, “Tropical seaweed farming trends: Problems and opportunities,” in Tropical Seaweed Farming Trends, Problems and Opportunities: Focus on Kappaphycus and Eucheuma of Commerce, A. Q. Hurtado, A. T. Critchley, and I. C. Neish, Eds. Cham, Switzerland: Springer, 2017, pp. 1–450.